Intrinsic origin of atrioventricular nodal functional properties in rabbits

The goal of the present study was to document the intrinsic origin of atrioventricular nodal functional properties (recovery time, facilitation, and fatigue) in two groups of six superfused isolated rabbit heart preparations. These properties were determined from recovery curves (A2H2 versus H1A2 intervals) obtained with previously defined periodic premature stimulation sequences performed before and after autonomic blockade demonstrated effective with the injection of agonists. In group 1 preparations, the muscarinic cholinergic and beta-adrenergic receptors were blocked with atropine (1 mg/L) and propranolol (1 mg/L), respectively. The blockade reduced the facilitation and increased the fatigue by the direct membranous effect of propranolol. To avoid this effect in group 2 preparations, propranolol was replaced by sotalol (5 mg/L), which in combination with atropine altered neither the presence nor the magnitude of the functional properties. These results show that the origin of the three nodal functional properties is independent from the autonomic nervous system.