Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging based on lateral shearing
Applied Optics
|September 24, 2016
Summary
This study introduces a compact dual-wavelength microscopy technique for quantitative phase imaging. The method uses a single glass plate for vibration-resistant, extended-range imaging of microstructures.
Area of Science:
- Optics and Photonics
- Microscopy
- Interferometry
Background:
- Quantitative phase imaging is crucial for label-free microscopy.
- Traditional methods can be complex and sensitive to vibrations.
Purpose of the Study:
- To develop a compact and vibration-resistant dual-wavelength digital holographic microscopy system.
- To achieve quantitative phase imaging using lateral shearing interferometry.
Main Methods:
- A common-path digital holographic microscopy setup utilizing a single parallel glass plate.
- Employing dual wavelengths (532 nm and 632.8 nm) for illumination.
- Generating lateral shear through front and back surface reflections of the glass plate.
Main Results:
- Successful reconstruction of phase distribution using a synthetic wavelength (3339.8 nm).
- Demonstrated feasibility on laser-ablated pit, groove, and staircase specimens.
- The system exhibits compactness and resistance to vibrations.
Conclusions:
- The proposed dual-wavelength common-path microscopy is a viable technique for quantitative phase imaging.
- The compact design offers advantages in stability and measurement range.
- This method is suitable for inspecting microstructures.
