Related Experiment Video
Updated: Mar 14, 2026

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
Published on: February 8, 2014
Extending depth of field for hybrid imaging systems via the use of both dark and dot point spread functions
Abstract:
In this paper, we propose one method based on the use of both dark and dot point spread functions (PSFs) to extend depth of field in hybrid imaging systems. Two different phase modulations of two phase masks are used to generate both dark and dot PSFs. The quartic phase mask (QPM) is used to generate the dot PSF. A combined phase mask between the QPM and the angle for generating the dark PSF is investigated. The simulation images show that the proposed method can produce superior imaging performance of hybrid imaging systems in extending the depth of field.
Related Concept Videos
Super-resolution Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

