Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR

CRISPR

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

50 Jahre (und mehr) Humangenetik/Medizinische Genetik in Berlin.

Medizinische Genetik : Mitteilungsblatt des Berufsverbandes Medizinische Genetik e.V·2026

Same author

A developmental shift in glucocorticoid receptor expression preserves glucocorticoid sensitivity in the adult suprachiasmatic nucleus.

PLoS biology·2026

Same author

A 3D Human Bone and Bone Marrow-on-a-Chip Model for In Vitro Bone Remodeling and Immune Cell Maintenance.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

HiFi sequencing accurately identifies clinically relevant variants in paralogous genes.

American journal of human genetics·2026

Same author

Organ formation in early human embryos captured in spatial cell atlas.

Nature·2026

Same author

Temporal constraints on enhancer usage shape the regulation of limb gene transcription.

Nature communications·2026

Search research articles

Related Experiment Video

Updated: Mar 14, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

CRISPR/Cas9 Genome Editing in Embryonic Stem Cells.

Guillaume Andrey¹, Malte Spielmann²

¹Development and Disease Group, Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, Berlin, 14195, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|September 25, 2016

Summary

CRISPR/Cas9 technology enables precise genome engineering in embryonic stem cells (ESCs). This protocol facilitates the creation of diverse mutations for studying gene function and modeling congenital diseases.

Keywords:

CRISPR Cas9 ES cells Genome engineering Indels Mouse Point mutation Structural variants

More Related Videos

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Related Experiment Videos

Last Updated: Mar 14, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Targeted mutagenesis is crucial for understanding DNA segment function.
CRISPR/Cas9 technology is increasingly used for functional genomics, offering advantages over traditional methods.
CRISPR/Cas9 enables various engineered mutations, including point mutations and large chromosomal rearrangements.

Purpose of the Study:

To present a protocol for engineering Embryonic Stem Cells (ESCs) with specific mutations using CRISPR/Cas9.
To demonstrate the utility of CRISPR/Cas9 for in vivo modeling of congenital mutations.
To enable functional interrogation of DNA sequences through precise genetic modification.

Main Methods:

Transfection of custom-made CRISPR/Cas9 vectors into ESCs.
Utilizing CRISPR/Cas9 technology for targeted genome editing.
Engineering a wide range of mutations, from point mutations to chromosomal rearrangements.

Main Results:

Successful engineering of ESCs with desired mutations.
Demonstration of CRISPR/Cas9's capability to create diverse genetic alterations.
Establishment of a protocol for efficient gene editing in ESCs.

Conclusions:

CRISPR/Cas9 technology provides a powerful tool for functional genome studies.
The presented protocol enables precise in vivo modeling of congenital mutations.
This method facilitates the functional interrogation of DNA sequences in ESCs.