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Implication of activation of intraperitoneal macrophages with bio-degradable microspheres
1Department of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Japan.
Abstract:
To estimate the possibility that biological degradable starch microspheres (DMS) activate abdominal or intraperitoneal macrophages (IMP), two sizes of DMS (Spherex, Pharmacia, Sweden) were injected into the peritoneum of the ICR mice of 4 to 8 weeks of age. Three days after the injection, peritoneal fluid was collected and incubated for one hour at 37 degrees C under 5% CO2. The cells which adhered to the petri dish were IMP, to which DMS was added for 18 hrs. The cultured IMP were observed by scanning electron microscope (SEM) and the ratio of the active type to the total number of IMP was counted as an index of the effect of DMS to IMP. The activation effect of DMS on the incubated IMP was significant in the group which was cultured with 2 microns DMS after the 45 microns DMS injection. That indicated the possible DMS function as a potential IMP activating factor (MAF).
Insights
Biodegradable starch microspheres (DMS) may activate intraperitoneal macrophages (IMP). Smaller DMS (2 microns) significantly enhanced IMP activation after larger DMS (45 microns) injection in mice.
Area of Science:
- Immunology
- Biomaterials Science
- Cell Biology
Background:
- Intraperitoneal macrophages (IMP) are crucial immune cells in the abdominal cavity.
- Biodegradable starch microspheres (DMS) are novel biomaterials with potential biomedical applications.
- Understanding the interaction between biomaterials and macrophages is vital for predicting in vivo responses.
Purpose of the Study:
- To investigate the potential of biodegradable starch microspheres (DMS) to activate intraperitoneal macrophages (IMP).
- To evaluate the influence of DMS size on macrophage activation.
- To determine if DMS can act as a macrophage activating factor (MAF).
Main Methods:
- Two sizes of DMS (2 and 45 microns) were injected into the peritoneum of ICR mice.
- Peritoneal fluid was collected, and adhered cells identified as IMP.
- IMP were incubated with DMS, and activation was assessed using scanning electron microscopy (SEM) and cell counting.
Main Results:
- Significant activation of IMP was observed when incubated with 2-micron DMS following a 45-micron DMS injection.
- The ratio of active to total IMP served as an index for DMS-induced activation.
- SEM imaging provided visual confirmation of DMS interaction with IMP.
Conclusions:
- Biodegradable starch microspheres (DMS) demonstrate a potential to activate intraperitoneal macrophages (IMP).
- DMS size plays a role in modulating macrophage activation.
- DMS may function as a potential macrophage activating factor (MAF).