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Updated: Dec 28, 2025
Author Spotlight: Advancements in Understanding and Combatting Shigella Infections
Published on: February 9, 2024
Shigellosis murine model established by intraperitoneal and intranasal route of administration: a comparative
Drashya Sharma1, Bhrugu Yagnik1, Ruma Baksi2
1Department of Cell and Molecular Biology, B.V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Ahmedabad, 380054, Gujarat, India; BRD School of Bioscience, Sardar Patel University, Vallabh Vidhyanagar, 388120, Gujarat, India.
Abstract:
Shigellosis, a major cause of mortality and morbidity, requires development of effective intervention strategy for which animal model mimicking human pathology is essential. Among various animal models for shigellosis, mice being more convenient have been used wherein intraperitoneal and intranasal routes are preferred. With the aim to comprehend the comparative pathophysiological indicators, we have examined relatively high and low dose of Shigella flexneri administered through intraperitoneal and intranasal routes in mice. Characterization of these two models along with the resulting pathophysiology of shigellosis adds to our understanding and offers suitable models appropriate to the objectives of the study.
Insights
Developing effective shigellosis interventions requires accurate animal models. This study compares Shigella flexneri administration routes and doses in mice to better understand disease pathophysiology for improved research.
Area of Science:
- Infectious Diseases
- Microbiology
- Animal Models
Background:
- Shigellosis is a significant global health concern causing high mortality and morbidity.
- Effective intervention strategies for shigellosis necessitate reliable animal models that accurately mimic human pathology.
- Mice are a convenient animal model for shigellosis research, with intraperitoneal and intranasal routes being commonly employed.
Purpose of the Study:
- To comparatively analyze pathophysiological indicators of shigellosis in mice.
- To evaluate the impact of different doses of Shigella flexneri administered via intraperitoneal and intranasal routes.
- To characterize and validate mouse models for shigellosis research.
Main Methods:
- Administration of high and low doses of Shigella flexneri to mice.
- Utilizing both intraperitoneal and intranasal routes for bacterial administration.
- Comparative examination of resulting pathophysiological indicators.
Main Results:
- Characterization of distinct pathophysiological profiles based on administration route and dosage.
- Identification of key indicators for assessing shigellosis severity in the mouse model.
- Demonstration of differential host responses to Shigella flexneri infection.
Conclusions:
- The study provides a comparative understanding of Shigella flexneri pathophysiology in mice.
- The characterized models offer suitable options for shigellosis research, tailored to specific study objectives.
- This research contributes to the development of more effective shigellosis intervention strategies.
