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Updated: Mar 14, 2026

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

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Published on: June 25, 2013

Smc5/6 complex regulates Sgs1 recombination functions.

Marcelino Bermúdez-López¹, Luis Aragon²

¹Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College, London, W12 0NN, UK.

Current Genetics

|September 25, 2016

Summary

The Smc5/6 complex recruits and regulates the STR complex (Sgs1-Top3-Rmi1) via SUMOylation, crucial for DNA repair and genome stability in yeast.

Keywords:

DNA damage Homologous recombination SUMOylation Sgs1 Smc5/6 complex Top3

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

RecQ helicases, including yeast Sgs1, are vital for maintaining genome stability.
Sgs1 functions within the STR complex (Sgs1-Top3-Rmi1) during DNA repair.
Mechanisms of Sgs1 recruitment and regulation at DNA damage sites are not fully understood.

Purpose of the Study:

To elucidate the regulatory role of the Smc5/6 complex in the function of the STR complex.
To detail how Smc5/6 influences Sgs1-mediated DNA repair processes.

Main Methods:

Investigated the interaction between Smc5/6 and STR complex components.
Analyzed the impact of Mms21-dependent SUMOylation on STR complex activity.
Studied DNA repair pathways involving Sgs1 and Smc5/6 in budding yeast.

Main Results:

Smc5/6 complex acts as a platform for STR complex recruitment.
Mms21-dependent SUMOylation by Smc5/6 regulates STR complex functions.
STR complex assembly is independent of the Smc5/6 complex.

Conclusions:

Smc5/6 complex is a key regulator of STR complex function during DNA repair.
SUMOylation is a critical mechanism by which Smc5/6 controls Sgs1 activity.
This interaction is essential for maintaining genome stability.