Magnetic solid-phase extraction based on graphene oxide for the determination of lignans in sesame oil
Linxia Wu1, Li Yu2, Xiaoxia Ding3
1Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China.
Abstract:
Graphene oxide was fabricated by a simple method and applied to magnetic solid-phase extraction. In a pretreatment procedure before the sesamol, sesamin and sesamolin in sesame oil were detected by high performance liquid chromatography. Several parameters affecting the extraction efficiency were investigated, including the type and volume of desorption solvent, desorption time and the amount of sorbent. Under the optimized conditions, the detection limits of sesamol, sesamin, and sesamolin were 0.05μg/g, 0.02μg/g, and 0.02μg/g, respectively. The limits of quantification were all 0.2μg/g. The average recoveries of sesamol, sesamin, and sesamolin were 84.55%, 85.47%, 86.83%, respectively and their relative standard deviations were 1.23%, 1.33%, and 0.84%, respectively.
