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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

100.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Related Experiment Video

Updated: Mar 14, 2026

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
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Next-Generation Sequencing in Cancer Diagnostics.

Christopher L Corless1

  • 1Knight Diagnostic Laboratories, Oregon Health & Science University, Portland, Oregon.

The Journal of Molecular Diagnostics : JMD
|September 25, 2016
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) platforms are crucial for identifying and confirming somatic variants. This commentary emphasizes their importance in current genomic research.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Somatic variants are key to understanding cancer development.
  • Accurate detection of these variants is essential for personalized medicine.

Purpose of the Study:

  • To highlight the utility of next-generation sequencing (NGS) platforms.
  • To underscore the importance of NGS in somatic variant detection and verification.

Main Methods:

  • Review of current literature on NGS applications.
  • Discussion of Misyura et al.'s findings on somatic variant analysis.

Main Results:

  • NGS platforms offer high sensitivity and specificity for variant detection.
  • Verification of somatic variants is robust with advanced sequencing technologies.

Conclusions:

  • Next-generation sequencing is indispensable for accurate somatic variant analysis.
  • The findings support the widespread adoption of NGS in clinical and research settings.