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Updated: Mar 14, 2026

Isolation and Identification of Bacterial Strains from Skin of Terrestrial Amphibians
Published on: June 17, 2025
Roseovarius lacus sp. nov., isolated from Yuncheng Saline Lake, China
Jiafeng Pan1,2, Zhen Yu3, Jia Tang2
1College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
Abstract:
Strain GSS12T, a Gram-negative, aerobic, non-flagellated, ovoid- to rod-shaped (0.5-0.7 × 0.9-3.0 µm) bacterium, was isolated from Yuncheng Saline Lake, China. Growth occurred with 0.5-16.0 % (w/v) NaCl (optimum 4.5 %), at pH 5.0-10.0 (optimum pH 6.0-6.5) and at 10-50 °C (optimum 37 °C). The major fatty acids (>5.0 %) found in GSS12T were summed feature 8 (72.2 %), C16:0 (9.0 %) and C18:1 ω7c 11-methyl (6.4 %). The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequences showed that strain GSS12T forms a stable clade with species of the genus Roseovarius, being related to R. pacificus 81-2T and R. litoreus GSW-M15T with 97.9 and 96.7 % of sequence similarity, respectively. The DNA-DNA relatedness values between strain GSS12T and R. pacificus 81-2T and R. halotolerans HJ50T were low (36 and 29 %, respectively). The phenotypic, physiological, biochemical and genetic characteristics support the assignment of strain GSS12T to the genus Roseovarius and represent a novel species. The name Roseovarius lacus sp. nov. is proposed, with strain GSS12T (=KCTC 52185T =MCCC 1K02302T) as the type strain.
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