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Updated: Mar 14, 2026

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
Published on: April 17, 2016
Chemical Secretory Pathway Modulation in Plant Protoplasts
Francesca De Marchis1, Andrea Pompa1, Michele Bellucci2
1Institute of Biosciences and Bioresources, Research Division of Perugia, National Research Council, 06128, Perugia, Italy.
Abstract:
The classical Golgi pathway is not the only mechanism for vacuolar protein transport in plants because alternative transport mechanisms have been described. The existence of these alternative pathways can be demonstrated using several chemicals and here we describe the use of brefeldin A (BFA), endo-β-N-acetylglucosaminidase H (Endo-H), and tunicamycin, on isolated tobacco leaf protoplasts. Two main methods are illustrated in this chapter, protoplast pulse-chase followed by protein immunoprecipitation, and protoplast immunofluorescence.
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