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Related Experiment Video

Updated: Mar 14, 2026

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Published on: May 22, 2020

Cell surface cathepsin G activity differs between human natural killer cell subsets.

Adriane Penczek¹, Marcin Sienczyk², Christian Rainer Wirtz¹

¹Department of Neurosurgery, Ulm University Medical Center, Ulm, Germany.

Immunology Letters

|September 27, 2016

Summary

This summary is machine-generated.

Natural killer (NK) cells play a key role in immunity. Researchers found that the cell surface protease cathepsin G (CatG) levels vary across different NK cell subsets, enabling their differentiation.

Keywords:

NK cells,activity-based probes cathepsin G

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A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

Published on: August 9, 2017

Area of Science:

Immunology
Cell Biology
Protease Function

Background:

Natural killer (NK) cells are crucial for innate immunity, targeting viral-infected and tumor cells.
NK cell activation is regulated by the presence or absence of major histocompatibility complex class I (MHC I) molecules on target cells.
The protease cathepsin G (CatG) is known to bind to NK cell surfaces, but its distribution among NK cell subsets remains unclear.

Purpose of the Study:

To investigate the differential expression and activity of cathepsin G (CatG) on various natural killer (NK) cell subsets.
To establish CatG as a potential marker for distinguishing between distinct NK cell populations.

Main Methods:

Utilized flow cytometry to quantify CatG protein levels on NK cell surfaces.
Employed the activity-based probe MARS116 to assess CatG enzymatic activity.
Analyzed CatG distribution across different NK cell subsets.

Main Results:

Demonstrated significant differences in cell surface CatG levels among distinct NK cell subsets.
Validated MARS116 as a reliable tool for detecting cell surface CatG activity.
Showcased the potential of CatG as a distinguishing feature for NK cell subsets.

Conclusions:

Cell surface CatG levels are heterogeneous across different NK cell populations.
The activity-based probe MARS116 serves as a novel reporter for cell surface CatG activity.
CatG expression and activity can be utilized to differentiate between distinct NK cell subsets, advancing NK cell subset characterization.