Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Folding01:22

Protein Folding

129.8K
Overview
129.8K
Protein Folding01:25

Protein Folding

12.1K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
12.1K
Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

65.8K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
65.8K
Adaptability of Cytoskeletal Filaments01:12

Adaptability of Cytoskeletal Filaments

6.5K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
6.5K
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.8K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.8K
Mechanisms of Membrane-bending01:15

Mechanisms of Membrane-bending

3.6K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular structure of the third immunoglobulin domain (Ig3) of human Muscle-Specific kinase (MuSK).

Journal of structural biology·2026
Same author

Selection of Functional Glycoforms in Anti-SARS-CoV-2 Human IgG1 Monoclonal Antibodies by FcγRIIIa Affinity Chromatography and Mass Spectrometry.

Journal of medicinal chemistry·2026
Same author

A novel biomarker of human exposure to Aedes albopictus based on the Ag5-3 salivary protein from the tiger mosquito.

Parasites & vectors·2025
Same author

A digital repository of samples from arthropod vectors.

Pathogens and global health·2025
Same author

<i>Spiroplasma</i> impairs testes gene expression in <i>Glossina fuscipes fuscipes</i>.

bioRxiv : the preprint server for biology·2025
Same author

Design of multi-target peptide modulators for protein chaperone networks.

Structure (London, England : 1993)·2025
Same journal

Editorial: Epigenetic and genetic mechanisms underlying cardiovascular diseases and neurodevelopmental disorders, volume II.

Frontiers in molecular biosciences·2026
Same journal

Integrated transcriptomic profiling reveals oncogenic pathways and chimeric transcripts in equine sarcoid lesions with predominant BPV1 detection.

Frontiers in molecular biosciences·2026
Same journal

Mesenchymal stem cells-derived extracellular vesicles as a novel drug delivery carrier: engineering strategies and clinical safety estimation.

Frontiers in molecular biosciences·2026
Same journal

Preparation and analysis of tobacco glycosides, and the relationship between glycoside aglycones and pyrolysis products: a review.

Frontiers in molecular biosciences·2026
Same journal

Peritoneal metastasis in pancreatic cancer: molecular mechanisms, microenvironmental remodeling, and emerging intraperitoneal interventions.

Frontiers in molecular biosciences·2026
Same journal

Insights from LC-MS-based cerebrospinal fluid metabolomics in tuberculous meningitis.

Frontiers in molecular biosciences·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

1.2K

Identifying and Visualizing Macromolecular Flexibility in Structural Biology.

Martina Palamini1, Anselmo Canciani1, Federico Forneris1

  • 1The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia Pavia, Italy.

Frontiers in Molecular Biosciences
|September 27, 2016
PubMed
Summary
This summary is machine-generated.

Structural biology now better visualizes molecular flexibility and dynamics using advanced techniques. These methods, including cryo-electron microscopy and time-resolved studies, are crucial for understanding biological regulation.

Keywords:
Small-angle scatteringX-ray crystallographycryo-electron microscopyensemblesmolecular recognitionnuclear magnetic resonanceprotein flexibilitystructural biology

More Related Videos

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.5K
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

10.4K

Related Experiment Videos

Last Updated: Mar 14, 2026

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

1.2K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.5K
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

10.4K

Area of Science:

  • Structural Biology
  • Biophysics
  • Biochemistry

Background:

  • Conventional structural biology methods (crystallography, NMR, cryo-electron microscopy) often lack detail on molecular flexibility and dynamics.
  • Flexibility is critical for macromolecular function, ligand interactions, and biological regulation.
  • Studying dynamic biological systems like large assemblies and membrane proteins is challenging.

Purpose of the Study:

  • To review how recent technological advancements in structural biology enable the investigation of molecular flexibility and dynamics.
  • To highlight the integration of modern biophysical and computational approaches for studying dynamic systems.
  • To demonstrate the utility of these methods in understanding biological processes.

Main Methods:

  • Time-resolved studies
  • Solution X-ray scattering
  • Advanced cryo-electron microscopy detectors
  • Ensemble modeling
  • Selective particle picking in cryo-electron microscopy

Main Results:

  • Modern structural biology methods can now capture subtle molecular motions.
  • Technological advancements have expanded the scope of structural investigations beyond traditional NMR analysis.
  • Integration of methods allows for more feasible studies of dynamic biological systems.

Conclusions:

  • Current structural biology methods provide crucial data for visualizing macromolecular flexibility.
  • Understanding molecular dynamics is essential for comprehending the regulation of biological processes.
  • Advanced techniques are transforming the study of intrinsically disordered proteins and folding intermediates.