[Variability of Apo B-containing lipoprotein in the plasma of experimental atherosclerotic rabbits]

The effects of dietary cholesterol on plasma Apo B-containing lipoprotein was studied in 18 rabbits. When atherosclerosis was induced by dietary cholesterol, the cholesterol and peroxide lipids were increased in the intima of aortae (P less than 0.05). The increased plasma Apo B was positively correlated with hypercholesterolemia (r = 0.879, P less than 0.005). In the pre-stain lipoprotein electrophoresis and immunoelectrophoresis with Apo B antiserum, electrophoretic mobility of Apo B-containing lipoprotein was slower than those of the control rabbits and this band was wide and stained deeper. In Sephadex G-200 exclusion chromatography there was evidence showing that the reduction in electrophoretic mobility of Apo B-containing lipoprotein is due to the increase particle size.