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Th1, Th17, CXCL16 and homocysteine elevated after intracranial and cervical stent implantation
Yanyan Tang1, Yunfei Wei1, Ziming Ye1
1a Department of Neurology , The First Affiliated Hospital, Guangxi Medical University , Nanning , China.
Insights
Inflammation markers, including T-helper 1 (Th1) and T-helper 17 (Th17) cells, CXCL16, and homocysteine, change after cerebral stenting. These variations may play a role in post-stent inflammation.
Area of Science:
- Immunology
- Cardiovascular Science
- Neurology
Background:
- T-helper 1 (Th1) and T-helper 17 (Th17) cells are key drivers of inflammation.
- Cerebral stenting can trigger immune responses, but the roles of Th1, Th17, CXCL16, and homocysteine are not well understood.
Purpose of the Study:
- To analyze the dynamic changes of Th1, Th17 cells, CXCL16, and homocysteine in peripheral blood following cerebral stent implantation.
- To investigate the potential involvement of these factors in post-procedural inflammation.
Main Methods:
- Flow cytometry was used to quantify Th1 and Th17 cell proportions in peripheral blood mononuclear cells (PBMCs).
- Enzyme-linked immunosorbent assay (ELISA) measured serum levels of IFN-γ, IL-17, and CXCL16.
- Plasma homocysteine concentrations were determined using immunoturbidimetry.
Main Results:
- Th1 cells, CXCL16, and homocysteine levels increased at 3 days post-stenting, then decreased at 7 days and 3 months.
- Th17 cell frequency peaked at 3 days and subsequently decreased but remained elevated compared to baseline.
- Observed variations in Th1, Th17, CXCL16, and homocysteine suggest their implication in inflammation after intracranial and cervical stenting.
Conclusions:
- The study identifies dynamic changes in Th1, Th17, CXCL16, and homocysteine post-cerebral stenting.
- These factors may contribute to the inflammatory response following stent implantation.
- Understanding these markers could aid in developing targeted therapies and drug design for stenting procedures.
Abstract:
The presence of Th1 and Th17 cells has been observed as major inducers in inflammation and immune responses associated stenting. However, there is rare data on the impact of Th1, Th17, CXCL16 and homocysteine after cerebral stent implantation. Here, we performed the statistical analysis to first evaluate the variation of the Th17and Th1 cells and their related cytokines, CXCL16 and homocysteine in the peripheral blood of patients with cerebral stenting. The flow cytometry was used to detect the proportion of Th1 and Th17 cells in peripheral blood mononuclear cells (PBMCs). The enzyme-linked immunosorbent assay was used to measure the serum concentrations of IFN-γ, IL-17 and CXCL16. Plasma homocysteine was examined by immunoturbidimetry. The level of Th1, CXCL16 and homocysteine showed an increase at 3 d, followed by the continuous decrease at 7 d and 3 months. The frequency of Th17 cells increased to a peak at three days, and subsequently decreased with a higher level than baseline. Our data revealed that the variation in Th1, Th17, CXCL16 and homocysteine in peripheral blood of patients with stenting may be implicated in inflammation after intracranial and cervical stent implantation. A better understanding of these factors will provide help for further drug design and clinical therapy.
