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Related Experiment Videos

Multiple mRNAs encode peripherin, a neuronal intermediate filament protein.

F Landon1, M Lemonnier, R Benarous

  • 1Collège de France, Paris.

The EMBO Journal
|June 1, 1989
PubMed
Summary
This summary is machine-generated.

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Three distinct messenger RNAs (mRNAs) for peripherin, a neuronal intermediate filament protein (IFP), were identified. Alternative splicing of a single peripherin gene generates these mRNAs, leading to protein polymorphism.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Peripherin is a neuronal intermediate filament protein (IFP).
  • Understanding peripherin gene expression and protein variants is crucial for neuronal development and function.

Purpose of the Study:

  • To isolate and characterize cDNA clones specific for peripherin.
  • To investigate the molecular basis of peripherin heterogeneity in neuronal cells.

Main Methods:

  • Isolation of cDNA clones from a murine neuroblastoma cell library using immunoscreening.
  • Sequence analysis of cDNA clones and comparison with known IFP cDNAs.
  • S1 mapping experiments to identify distinct mRNAs.
  • In vitro translation of poly(A)+ RNA to detect peripherin protein products.

Related Experiment Videos

Main Results:

  • Three cDNA clones (1.6, 1.2, and 0.6 kbp) specific for peripherin were isolated.
  • Sequence analysis revealed variations in cDNA clones 3u and 5b compared to cDNA 5g, suggesting alternative splicing.
  • S1 mapping confirmed the existence of three distinct peripherin mRNAs.
  • In vitro translation demonstrated three peripherin protein products (58, 61, and 56 kDa), with the 61 kDa product translated from the 3u-selected RNA.

Conclusions:

  • Alternative splicing of a unique peripherin gene generates three distinct mRNAs.
  • This alternative splicing leads to the production of multiple peripherin protein isoforms, contributing to protein polymorphism.
  • The identified peripherin variants may play specific roles in neuronal function.