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Updated: Mar 14, 2026
One-step Extraction and Zymographic Analysis of Bacterial Gelatinases
Published on: August 1, 2025
Structural analysis of macromolecular levan produced by Bacillus megaterium GJT321 based on enzymatic method
Xiaomeng Yu1, Liyan Li2, Jingliang Zhang3
1College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China.
Abstract:
Extracellular polysaccharide (EPS) produced by Bacillus megaterium GJT321 was isolated from fermentation broth and further purified by gel filtration chromatography. The molecular weight of EPS was estimated as 1946kDa by gel permeation chromatography (GPC), so this EPS was determined as macromolecular polysaccharide. Fourier transform infrared spectrometry (FT-IR) showed that the kind of heterocyclic compound composing EPS was furanose. The structural characteristics of EPS were investigated by means of enzymatic method, electrospray ionization mass spectrometry (ESI-MS) and NMR spectra analysis. The structure elucidation of the EPS was accomplished, and it was β - (2, 6) -D- fructofuranose, namely levan.
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