Related Experiment Video
Updated: Mar 14, 2026

08:21
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
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Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria
1Synthetic Biology Group, Institute Pasteur, Paris, 75015, France.
Methods in Molecular Biology (Clifton, N.J.)
|September 28, 2016
Abstract:
Clonetegration is a method for site-specific insertion of DNA into prokaryotic chromosomes, based on bacteriophage integrases. The method combines DNA cloning/assembly and chromosomal integration into a single step, providing a simple and rapid strategy for inserting DNA sequences into bacterial chromosomes.

