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Establishing a Device for Sleep Deprivation in Mice
Published on: September 22, 2023
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Melatonin modulates adiponectin expression on murine colitis with sleep deprivation
Tae Kyun Kim1, Young Sook Park1, Haing-Woon Baik1
1Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
World Journal of Gastroenterology
|September 28, 2016
Summary
Melatonin treatment improved colitis in mice by increasing adiponectin expression and reducing inflammatory cytokines, especially under sleep deprivation. This highlights melatonin
Area of Science:
- Gastroenterology
- Immunology
- Sleep Medicine
Background:
- Murine colitis models are crucial for understanding inflammatory bowel disease.
- Sleep deprivation is a known stressor that can exacerbate inflammatory conditions.
- Adiponectin plays a role in regulating inflammation.
Purpose of the Study:
- To investigate the effect of melatonin on adiponectin expression in colonic tissue during murine colitis.
- To examine systemic cytokine expression in response to melatonin treatment and sleep deprivation in colitis models.
- To determine the synergistic effects of melatonin and adiponectin in managing colitis.
Main Methods:
- C57BL/6 mice were induced with colitis using 2% DSS.
- Experimental groups included control, colitis, colitis with melatonin, colitis with sleep deprivation (SD), and colitis with SD and melatonin.
- Adiponectin expression was assessed via immunohistochemistry and Western blot; serum cytokines (IL-6, IL-17) were measured by ELISA.
Main Results:
- Sleep deprivation worsened DSS-induced colitis and weight loss.
- Melatonin treatment alleviated mucosal injury and improved survival rates.
- Adiponectin expression decreased in colitis, particularly with SD, but was restored by melatonin. IL-6 and IL-17 levels decreased with melatonin treatment.
Conclusions:
- Melatonin modulates adiponectin expression in colonic tissue during colitis.
- Melatonin and adiponectin exhibit synergistic anti-inflammatory effects in colitis, particularly when combined with sleep deprivation.
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