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Aptamer-Based Technologies in Foodborne Pathogen Detection.

Jun Teng1, Fang Yuan2, Yingwang Ye1

  • 1College of Food Science and Engineering, Hefei University of Technology, Hefei China.

Frontiers in Microbiology
|September 28, 2016
PubMed
Summary

Aptamers, which are DNA or RNA molecules, offer powerful tools for detecting pathogens and screening biomolecules. Aptamer-based biosensors show great promise for public health and food safety applications, though challenges remain.

Keywords:
SELEXaptamer-based biosensorsaptamersbacterial pathogen detectionbiomolecular screeningdissociation constantshigh affinityligands

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biosensor Technology

Background:

  • Aptamers are single-stranded DNA or RNA ligands selected via systematic evolution of ligands by exponential enrichment (SELEX).
  • Their specific target recognition and binding capabilities are crucial for applications in pathogen detection and biomolecular screening.
  • Rapid, sensitive, and efficient detection technologies are vital for assessing risks associated with pathogen infections and food contamination.

Purpose of the Study:

  • To review the advancements in aptamer-based approaches for pathogen detection.
  • To cover the development of aptamer-based biosensors, including optical, electrochemical, and lateral flow assays.
  • To discuss the applications of these technologies in pathogen detection and biomolecular screening.

Main Methods:

  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX), including whole-cell SELEX and Genomic SELEX.
  • Development of various aptamer-based biosensors: optical (fluorescence, surface plasmon resonance), electrochemical, and lateral chromatography test strips.
  • Application of these biosensors for pathogen detection and biomolecular screening.

Main Results:

  • Aptamer-based approaches have shown significant potential in pathogen detection and biomolecular screening.
  • Diverse aptamer-based biosensors have been developed, offering sensitive and specific detection methods.
  • Progress has been made in applying these biosensors for real-world applications in public health and food safety.

Conclusions:

  • Aptamers and aptamer-based biosensors represent a promising field for pathogen detection and biomolecular screening.
  • Continued research and development are necessary to overcome existing challenges and fully realize their potential.
  • These technologies are critical for improving public health and food safety surveillance.