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Updated: Mar 14, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
A genomic approach for isolating chloroplast microsatellite markers for Pachyptera kerere (Bignoniaceae)
Jessica N C Francisco1, Alison G Nazareno1, Lúcia G Lohmann1
1Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo (USP), Rua do Matão 277, 05508-090 São Paulo, São Paulo, Brazil.
Premise Of The Study:
In this study, we developed chloroplast microsatellite markers (cpSSRs) for Pachyptera kerere (Bignoniaceae) to investigate the population structure and genetic diversity of this species.
Methods And Results:
We used Illumina HiSeq data to reconstruct the chloroplast genome of P. kerere by a combination of de novo and reference-guided assembly. We then used the chloroplast genome to develop a set of cpSSRs from intergenic regions. Overall, 24 primer pairs were designed, 21 of which amplified successfully and were polymorphic, presenting three to nine alleles per locus. The unbiased haploid diversity per locus varied from 0.207 (Pac28) to 0.817 (Pac04). All but one locus amplified for all other taxa of Pachyptera.
Conclusions:
The markers reported here will serve as a basis for studies to assess the genetic structure and phylogeographic history of Pachyptera.
