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Analysis of IncF plasmids evolution: nucleotide sequence of an IncFIII replication region
J Lopez1, P Crespo, J C Rodriguez
1Departamento de Biología Molecular, Facultad de Medicina, Santander, Spain.
Gene
|May 15, 1989
Abstract:
We determined the nucleotide sequence of RepFIII, the IncFIII replication region of the plasmid pSU316. Our data confirmed that RepFIII belongs to the RepFIIA family. The comparison of the nucleotide sequences from several RepFIIA-family plasmids revealed that pSU316 and R1 replicons are almost identical (96% similarity). Most of the differences between them are clustered in the incompatibility determinant. Analysis of the rest of the sequence suggested that the divergence between R1 and pSU316 replicons is very recent.