Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Presentation and Tumour Burden in Head and Neck Sarcomas: Implications for Early Diagnosis and Referral.

Cancers·2026
Same author

Imaging of High-Risk Neuroblastoma: Recommendations From SIOPEN Radiology and Nuclear Medicine Specialty Committees.

Pediatric blood & cancer·2026
Same author

FibreCastML: an open web platform for predicting electrospun nanofibre diameter distributions for biomedical applications.

Frontiers in bioengineering and biotechnology·2026
Same author

Multidisciplinary team interpretation performance for indeterminate bone uptake on PSMA PET during prostate cancer staging: Comparison with PROMISE criteria.

European journal of nuclear medicine and molecular imaging·2026
Same author

Efficacy of radiotherapy and role of adjunctive immunochemotherapy in POEMS syndrome with solitary plasmacytoma.

Blood advances·2026
Same author

Clinical features and treatment outcomes of POEMS-associated Castleman disease.

British journal of haematology·2026

Related Experiment Video

Updated: Mar 14, 2026

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

12.5K

Self-assembling peptide hydrogel for intervertebral disc tissue engineering.

Simon Wan1, Samantha Borland2, Stephen M Richardson3

  • 1School of Materials, University of Manchester, Manchester M13 9PL, UK.

Acta Biomaterialia
|November 6, 2016
PubMed
Summary

Self-assembling peptide hydrogels show promise for treating degenerative disc disease by serving as scaffolds for cell-based therapies. These hydrogels support cell viability and promote the regeneration of nucleus pulposus tissue, offering a potential solution for lower back pain.

Keywords:
Cell-based therapyDegenerative disc diseaseLow back painNucleus pulposusRegenerative medicineSelf-assembling peptide hydrogel

More Related Videos

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
07:52

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Published on: June 13, 2018

10.8K
Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility
05:24

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Published on: September 6, 2024

1.8K

Related Experiment Videos

Last Updated: Mar 14, 2026

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

12.5K
Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
07:52

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Published on: June 13, 2018

10.8K
Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility
05:24

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Published on: September 6, 2024

1.8K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Degenerative disc disease (DDD) is a major cause of lower back pain (LBP), with limited treatment options.
  • Cell-based therapies offer a promising approach for regenerating intervertebral disc (IVD) tissue.
  • Self-assembling peptide hydrogels (SAPHs) are advanced biomaterials with tunable properties for tissue engineering.

Purpose of the Study:

  • To investigate a de novo self-assembling peptide hydrogel (SAPH) as a cell carrier and scaffold for nucleus pulposus (NP) tissue engineering.
  • To evaluate the SAPH's potential for treating degenerative disc disease and alleviating lower back pain.

Main Methods:

  • Characterization of SAPH mechanical properties and injectability using oscillatory rheology.
  • 3D culture of nucleus pulposus cells (NPCs) within the SAPH scaffold.
  • Assessment of NP cell phenotype restoration via gene expression analysis (KRT8, KRT18, FOXF1).
  • Evaluation of cell viability and extracellular matrix (ECM) deposition (aggrecan, type II collagen).

Main Results:

  • The SAPH exhibited mechanical properties comparable to native human NP tissue and was deemed injectable.
  • NPCs cultured in the SAPH demonstrated restored NP-specific gene expression, indicating phenotype recovery.
  • High cell viability was maintained throughout the culture period.
  • Significant deposition of aggrecan and type II collagen was observed, essential components of NP ECM.

Conclusions:

  • The de novo SAPH functions effectively as a cell delivery system and scaffold for nucleus pulposus tissue engineering.
  • SAPHs hold significant potential for the development of cell-based therapies to treat degenerative disc disease.
  • This approach could lead to novel treatments for lower back pain associated with IVD degeneration.