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Computer assisted leukocyte adhesion measurement in intravital microscopy
H Zeintl1, F U Sack, M Intaglietta
1Department of Experimental Surgery, University of Heidelberg, FRG.
International Journal of Microcirculation, Clinical and Experimental
|July 1, 1989
Summary
Researchers developed a new model to quantify leukocyte-endothelium interactions in vivo. This method allows for reproducible measurements of leukocyte velocity and adhesion, aiding the study of postischemic injury.
Area of Science:
- Cardiovascular Research
- Immunology
- Microcirculation
Background:
- The role of leukocytes in postischemic injury remains unclear.
- Quantifying leukocyte-endothelium interactions in vivo is challenging.
Purpose of the Study:
- To develop a model for in vivo quantification of leukocyte-endothelium interaction in awake animals.
- To establish a reproducible method for assessing leukocyte behavior during postischemic injury.
Main Methods:
- Intravital microscopy was used to measure leukocyte velocity.
- Interactive digital image analysis was employed for quantification.
- An adhesion coefficient (AC) was calculated to characterize leukocyte-endothelium interaction.
- Automated image sampling reduced observer bias.
Main Results:
- The developed model allows for in vivo quantification of leukocyte-endothelium interaction.
- The automated procedure for image sampling yielded reproducible results.
- The method enables quantification of prophylactic and therapeutic interventions' effects.
Conclusions:
- The new model provides a reliable tool for studying leukocyte-endothelium interactions in postischemic injury.
- This technique facilitates research into the pathogenetic role of leukocytes.
- The method supports the evaluation of treatments targeting postischemic inflammation.