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Related Experiment Video

Updated: Mar 14, 2026

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

Published on: October 11, 2022

Virus Discovery Using Tick Cell Lines.

Lesley Bell-Sakyi¹, Houssam Attoui²

¹Head of The Tick Cell Biobank, The Pirbright Institute, Pirbright, Surrey, UK.

Evolutionary Bioinformatics Online

|September 29, 2016

Summary

High-throughput sequencing reveals diverse tick-borne viruses, but their nature remains unclear. Tick cell lines are crucial for discovering new viruses and determining if they are infectious or endogenous elements.

Keywords:

arbovirus endogenous viral element endogenous virus tick cell line

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Last Updated: Mar 14, 2026

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

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Published on: October 11, 2022

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Published on: April 6, 2022

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Published on: August 31, 2013

Area of Science:

Virology
Arthropod-borne diseases
Genomics

Background:

Ticks transmit pathogenic arboviruses, a known threat for decades.
Recent sequencing reveals a diverse range of tick-only viruses in ticks.
The origin and infectivity of these novel viruses are largely unknown.

Purpose of the Study:

To review advances in discovering tick-borne viruses.
To discuss the role of tick cell lines in virus discovery and characterization.
To highlight the potential of tick cell lines in identifying novel, replication-competent viruses.

Main Methods:

Review of recent scientific literature on tick-borne virus discovery.
Analysis of high-throughput sequencing data identifying viral sequences in ticks.
Discussion of tick cell culture techniques for virus isolation and propagation.

Main Results:

High-throughput sequencing has uncovered a wide array of viruses endemic to ticks.
Distinguishing between infectious viruses and endogenous viral elements remains a challenge.
Tick cell lines have proven effective in isolating and identifying novel viruses.

Conclusions:

Tick cell lines are invaluable tools for advancing tick-borne virus discovery.
Further research is needed to understand the biology and significance of newly identified tick viruses.
Tick cell lines aid in determining the replication competence and infectivity of potential tick-borne pathogens.