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Switching the Immunogenicity of Peptide Assemblies Using Surface Properties.

Yi Wen1,2, Amelia Waltman2, Huifang Han2

  • 1Biomedical Engineering Department, Duke University , Durham, North Carolina 27708, United States.

ACS Nano
|September 30, 2016
PubMed
Summary

Negative surface charge on supramolecular peptide biomaterials can completely abolish immune responses, preventing T cell and antibody reactions. This finding offers a strategy to control immunogenicity for in vivo applications.

Keywords:
biomaterialimmunogenicitynanofibersupramolecularvaccine

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Area of Science:

  • Biomaterials science
  • Immunology
  • Supramolecular chemistry

Background:

  • Supramolecular biomaterials from peptides and proteins are increasingly used in immunology and non-immunological applications.
  • Controlling the immunogenicity of these materials for in vivo use is crucial but not well understood.

Purpose of the Study:

  • To investigate the relationship between physicochemical properties of fibrillized peptide biomaterials and their immunogenicity.
  • To identify design rules for maximizing or minimizing immune responses to these materials.

Main Methods:

  • Investigated fibrillized peptide biomaterials with varying surface properties through co-assembly.
  • Assessed antibody and T cell responses in mice against materials displaying competent epitopes.
  • Analyzed the role of surface charge in antigen-presenting cell (APC) uptake and major histocompatibility complex class II (MHC-II) presentation.

Main Results:

  • Negative surface charge completely abolished antibody and T cell responses, even with a competent epitope.
  • Negative charge prevented APC uptake and subsequent epitope presentation via MHC-II.
  • Positive surface charge enhanced APC uptake of fibrillized peptides.

Conclusions:

  • Extensive negative surface charge on supramolecular peptide assemblies should be avoided in vaccine design.
  • Negative surface charge provides a strategy to switch off immunogenicity for non-immunological applications of these biomaterials.