Field-flow fractionation of macromolecules

Area of Science:

• Analytical Chemistry
• Separation Science
• Polymer Chemistry

Background:

• Field-flow fractionation (FFF) is a powerful separation technique.
• Its application extends to macromolecules, colloids, and cell-sized particles.
• This paper specifically addresses FFF for macromolecule analysis.

Purpose of the Study:

• To summarize the principles and characteristics of FFF relevant to macromolecule separation.
• To establish the basis of selectivity in FFF for macromolecules.
• To survey the general applicability of FFF to different classes of macromolecules.

Main Methods:

• Brief description of FFF principles.
• Summary of FFF characteristics for macromolecule separation efficacy.
• Classification of macromolecular substances into four groups based on molecular weight and solubility.
• Discussion of FFF subtechnique capabilities for each class.

Main Results:

• FFF demonstrates broad applicability to macromolecules.
• Selectivity in FFF is based on fundamental principles.
• Different FFF subtechniques are suited for specific macromolecular classes.
• Macromolecules are categorized by molecular weight (cutoff 10^6) and solubility (aqueous/organic).

Conclusions:

• FFF is a versatile and effective technique for macromolecule fractionation.
• Understanding FFF principles and characteristics is key to successful macromolecule separation.
• Tailored FFF subtechniques enable efficient fractionation across diverse macromolecular types.