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Polychelated cryogels: hemoglobin adsorption from human blood.

Kadir Erol1

  • 1a Department of Property Protection and Safety , Osmancık Ömer Derindere Vocational Higher School, Hitit University , Çorum , Turkey.

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|September 30, 2016
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Summary

This study optimized hemoglobin (Hb) adsorption using novel cryogels. The developed poly(HEMA-GMA)-Cu(II) cryogels achieved a high adsorption capacity of 193.8 mg Hb/g, demonstrating efficient Hb isolation from blood.

Keywords:
Cu(II) chelatingHemoglobinadsorptioncryogel

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Area of Science:

  • Biomaterials Science
  • Separation Science
  • Biochemistry

Background:

  • Hemoglobin (Hb) purification is critical for its applications.
  • Adsorption techniques using macroporous cryogels offer efficient separation pathways.
  • Poly(HEMA-GMA) cryogels functionalized with Cu(II) show promise for biomolecule adsorption.

Purpose of the Study:

  • To investigate and optimize hemoglobin adsorption onto Cu(II) immobilized poly(HEMA-GMA) cryogels.
  • To characterize the synthesized cryogel material and assess its adsorption performance.
  • To evaluate the feasibility of isolating hemoglobin from human blood using this method.

Main Methods:

  • Synthesis and characterization of Cu(II) immobilized poly(HEMA-GMA) cryogels using techniques like FT-IR, SEM, BET, and ICP-OES.
  • Optimization of Hb adsorption by varying parameters such as pH, interaction time, initial Hb concentration, temperature, and ionic strength.
  • Adsorption isotherm studies using Langmuir and Freundlich models.

Main Results:

  • The poly(HEMA-GMA)-Cu(II) cryogels exhibited excellent Hb adsorption capacity (193.8 mg Hb/g cryogel).
  • Optimal adsorption conditions were determined for maximizing Hb uptake.
  • Successful isolation of Hb from human blood was achieved with a capacity of 124.5 mg/g cryogel.
  • The adsorption process best fitted the Langmuir isotherm model.

Conclusions:

  • Cu(II) immobilized poly(HEMA-GMA) cryogels are highly effective for hemoglobin adsorption and purification.
  • The developed cryogel system provides a promising platform for efficient biomolecule separation from complex matrices like blood.
  • The Langmuir model accurately describes the hemoglobin adsorption behavior onto these cryogels.