Related Experiment Videos
Development and testing of a synthetic oligonucleotide probe for the detection of pathogenic Yersinia strains
M D Miliotis1, J E Galen, J B Kaper
1Department of Medicine, University of Maryland School of Medicine, Baltimore, 21201.
Journal of Clinical Microbiology
|July 1, 1989
Abstract:
A 24-base oligonucleotide probe specific for a region of the Yersinia enterocolitica virulence plasmid (pYV) associated with HEp-2 cell cytotoxicity and the Sereny reaction was constructed by using sequences flanking critical TnphoA insertions in a subcloned fragment of pYV. This probe, highly specific and sensitive for virulent yersiniae, detected pathogenic Y. enterocolitica isolates in artificially inoculated foods.