Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

28.2K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
28.2K
Telomeres and Telomerase02:41

Telomeres and Telomerase

7.8K
7.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Replication in Eukaryotes01:29

Replication in Eukaryotes

18.6K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
18.6K
Replication in Eukaryotes02:31

Replication in Eukaryotes

206.8K
Overview
206.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Elastin-like Polymer Targeting Vascular Endothelial Growth Factor Receptor-1 Reduces Survival in Serum-Starved Endothelial Cells.

Biochemical engineering journal·2025
Same author

Investigation Into the Role of Reductants and Cosubstrates in Lytic Polysaccharide Monooxygenase Thermothielavioides terrestris AA9E Binding to Cellulose by Single-Molecule Imaging.

Biotechnology and bioengineering·2025
Same author

Atomically Precise Nanoclusters as Co-Catalysts for Light-Activated Microswimmer Motility.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Super-Resolved Fluorescence Lifetime Imaging of Single Cy3 Molecules and Quantum Dots Using Time-Correlated Single Photon Counting with a Four-Pixel Fiber Optic Array Camera.

The journal of physical chemistry. A·2024
Same author

Interrupted DNA and Slow Silver Cluster Luminescence.

The journal of physical chemistry. C, Nanomaterials and interfaces·2023
Same author

Nanoscale imaging of quantum dot dimers using time-resolved super-resolution microscopy combined with scanning electron microscopy.

Nanotechnology·2023

Related Experiment Video

Updated: Mar 14, 2026

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions
11:21

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Published on: August 30, 2024

1.4K

Super-resolution optical microscopy study of telomere structure.

Mary Lisa Phipps1, Peter M Goodwin1, Jennifer S Martinez2

  • 1Los Alamos National Laboratory, Center for Integrated Nanotechnologies, P.O. Box 1663, Bikini Atoll Road, SM-30, Los Alamos, New Mexico 84545, United States.

Journal of Biomedical Optics
|September 30, 2016
PubMed
Summary

Telomeres, the protective caps on chromosome ends, may not always form a single t-loop structure. Super-resolution microscopy optimization revealed potential sample preparation issues that could affect telomere structure studies.

More Related Videos

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

13.6K
Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans
10:01

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

Published on: August 4, 2016

11.0K

Related Experiment Videos

Last Updated: Mar 14, 2026

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions
11:21

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Published on: August 30, 2024

1.4K
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

13.6K
Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans
10:01

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

Published on: August 4, 2016

11.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Microscopy

Background:

  • Telomeres protect chromosome ends from degradation and fusion.
  • Human telomeres consist of repetitive DNA sequences (TTAGGG) and proteins.
  • The t-loop model describes telomere end-structure, involving a displacement loop.

Purpose of the Study:

  • To investigate if telomeres adopt distinct configurations beyond the t-loop model.
  • To optimize super-resolution (SR) microscopy for studying telomere structure.
  • To assess the impact of sample preparation on telomere integrity.

Main Methods:

  • Optimization of super-resolution (SR) microscopy techniques.
  • Electron microscopy for visualizing telomere structures.
  • Analysis of chromatin and nucleosome integrity post-sample preparation.

Main Results:

  • Super-resolution microscopy is being optimized for telomere investigation.
  • Sample preparation methods may disrupt chromatin, causing nucleosome loss.
  • This disruption could limit the application of SR microscopy in telomere research.

Conclusions:

  • Telomere structure may be more complex than the t-loop model suggests.
  • Current sample preparation for SR microscopy may introduce artifacts.
  • Further refinement of microscopy techniques is needed for accurate telomere structure analysis.