First detection and molecular identification of Babesia gibsoni from Rhipicephalus sanguineus ticks

Babesia gibsoni was firstly identified in Rhipicephalus sanguineus ticks infested on dogs in Asia. A total of 1195 ticks collected from veterinary practitioners and pet clinics were examined for Babesia by nested-polymerase chain reaction assay based on the 18S small subunit ribosomal RNA (ssrRNA) gene. Babesia infection was detected in nymph, male and female ticks with an infection rate of 2.42%, 0.98% and 1.97%, respectively. Sequence and phylogenetic analysis revealed that these Babesia spp. were genetically affiliated to the same clades within the genospecies of B. gibsoni and B. vogeli, and can be discriminated from other genospecies of Babesia. Intra- and inter-species analysis based on the genetic distance (GD) values indicated a lower level in B. gibsoni (GD<0.011) compared with other genospecies of Babesia (GD>0.106) and out-group protozoa (GD>0.244). This study provides the first molecular evidence of B. gibsoni identified in nymphs of R. sanguineus ticks in Asia, and further confirms the detection of B. vogeli in nymphs and adults of R. sanguineus in northern Taiwan. The epizootiological significance of canine Babesia spp. transmitted by R. sanguineus tick needs to be further identified.