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Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
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E2F coregulates an essential HSF developmental program that is distinct from the heat-shock response.

Jian Li1, Laetitia Chauve1, Grace Phelps1

  • 1Department of Molecular Biosciences, Rice Institute for Biomedical Research, Northwestern University, Evanston, Illinois 60208, USA.

Genes & Development
|October 1, 2016
PubMed
Summary

Heat-shock factor 1 (HSF-1) regulates distinct gene networks for development and heat-shock response. Developmental HSF-1 activity requires E2F/DP binding to specific promoter elements, unlike the heat-shock response.

Keywords:
Caenorhabditis elegansE2F transcription factordevelopmentheat-shock factor (HSF)molecular chaperonesstress responsetranscription regulation

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Heat-shock factor (HSF) is crucial for stress resilience via the heat-shock response (HSR).
  • The role and regulation of HSF in metazoan development are not well understood.
  • HSF-1 is essential for development in Caenorhabditis elegans.

Purpose of the Study:

  • To characterize the genomic distribution and transcriptional activity of HSF-1 during C. elegans larval development.
  • To elucidate the distinct regulatory mechanisms governing the developmental HSF-1 program versus the HSR.
  • To identify the gene network regulated by HSF-1 during development.

Main Methods:

  • Genomic distribution analysis of HSF-1.
  • Transcriptional activity profiling during larval development.
  • Analysis of promoter elements and transcription factor binding (E2F/DP).

Main Results:

  • The developmental HSF-1 transcriptional program is distinct from the HSR.
  • Developmental HSF-1 activation depends on E2F/DP binding to GC-rich motifs and degenerate heat-shock elements (HSEs) near E2F-binding sites.
  • The HSR is independent of E2F/DP and relies on canonical HSEs.

Conclusions:

  • E2F and HSF-1 collaborate to regulate genes involved in protein biogenesis and anabolic metabolism during development.
  • Distinct promoter elements and transcription factor interactions dictate HSF-1 function in development versus stress response.
  • Understanding these distinct regulatory pathways is key to comprehending HSF-1's multifaceted roles.