ME-CAGEBIRD^r,X-CPMG-HSQMBC. A phase sensitive, multiplicity edited long range HSQC with absorptive line shapes

ME-CAGEBIRD^r,X-CPMG-HSMBC pulse sequence is a phase sensitive, carbon multiplicity edited 2D-experiment for detecting heteronuclear correlations originating from long-range ¹H, ¹³C-couplings, ⁿJ_CH. The presented method allows measurement of ⁿJ_CH-values as well as is capable of separating different carbon types in subspectra (¹³C/¹³CH₂ and ¹³CH/¹³CH₃) with minimal amount of cross talk i.e. cross peaks from wrong carbon multiplicity. Pure lineshapes and clean subspectra are achieved by utilizing CPMG in polarization transfer period, CRISIS-approach in multiplicity editing period and zero-quantum filtration. The obtained spectral properties together with simple setup of the experiment make ME-CAGEBIRD^r,X-CPMG-HSMBC a useful addition into synthetic organic chemistry oriented NMR-tool collection.