Surface chemistry from wettability and charge for the control of mesenchymal stem cell fate through self-assembled...

Self-assembled monolayers (SAMs) of alkanethiols on gold are highly controllable model substrates and have been employed to mimic the extracellular matrix for cell-related studies. This study aims to systematically explore how surface chemistry influences the adhesion, morphology, proliferation and osteogenic differentiation of mouse mesenchymal stem cells (mMSCs) using various functional groups (-OEG, -CH₃, -PO₃H₂, -OH, -NH₂ and -COOH). Surface analysis demonstrated that these functional groups produced a wide range of wettability and charge: -OEG (hydrophilic and moderate iso-electric point (IEP)), -CH₃ (strongly hydrophobic and low IEP), -PO₃H₂ (moderate wettability and low IEP), -OH (hydrophilic and moderate IEP), -NH₂ (moderate wettability and high IEP) and -COOH (hydrophilic and low IEP). In terms of cell responses, the effect of wettability may be more influential than charge for these groups. Moreover, compared to -OEG and -CH₃ groups, -PO₃H₂, -OH, -NH₂ and -COOH functionalities tended to promote not only cell adhesion, proliferation and osteogenic differentiation but also the expression of α_v and β₁ integrins. This finding indicates that the surface chemistry may guide mMSC activities through α_v and β₁ integrin signaling pathways. Model surfaces with controllable chemistry may provide insight into biological responses to substrate surfaces that would be useful for the design of biomaterial surfaces.