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Related Experiment Videos

Cell intercalation during notochord development in Xenopus laevis.

R Keller1, M S Cooper, M Danilchik

  • 1Department of Zoology, University of California, Berkeley 94720.

The Journal of Experimental Zoology
|August 1, 1989
PubMed
Summary

This study reveals Xenopus laevis notochord development involves three distinct phases of cell rearrangement and addition. Directional cell movements drive notochord morphogenesis during gastrulation and neurulation.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Morphogenesis

Background:

  • The notochord is a crucial embryonic structure providing skeletal support.
  • Understanding notochord development is key to comprehending vertebrate embryonic patterning.

Purpose of the Study:

  • To analyze the cellular events driving notochord morphogenesis in Xenopus laevis.
  • To elucidate the distinct phases and cellular behaviors involved in notochord formation during gastrulation and neurulation.

Main Methods:

  • Utilized scanning electron microscopy (SEM) for morphometric analysis of intact embryos.
  • Employed high-resolution time-lapse recordings of cell behavior in cultured explants.

Main Results:

  • Identified three phases of notochord development: cell boundary formation, cell intercalation, and cell flattening.

Related Experiment Videos

  • Observed cell intercalation leads to narrowing, thickening, and lengthening via directed cell movements.
  • Noted posterior cell addition contributes to notochord elongation.

    • Conclusions:

    • Notochord morphogenesis is orchestrated by distinct, coordinated cellular processes.
    • Directional cell movements and cell polarity are critical for cell intercalation and notochord shaping.
    • The study highlights the interplay of cell behavior, mechanics, and environment in embryonic development.