Telomere damage and redox status alterations in free-living passerines exposed to metals

Telomere length may reflect the expected life span and possibly individual quality. Environmental stressors are known to increase oxidative stress and accelerate telomere attrition: however the interactions between redox status and telomere dynamics are not fully understood. We investigated whether exposure to heavy metal pollution is associated with oxidative stress and telomere damage in two insectivorous passerines, the Great tit (Parus major) and the Pied flycatcher (Ficedula hypoleuca). We were also interested to know whether within-brood competition could influence the nestling redox status or telomere length. Breeding females and nestlings were sampled near the point pollution source and compared to birds in non-polluted control zone. We measured heavy metal concentrations, calcium, metallothioneins, telomere lengths and redox status (oxidative damage, and enzymatic and non-enzymatic antioxidants) in liver samples. Great tit nestlings in the polluted zone had significantly shorter telomeres compared to those in the unpolluted control zone. In addition, those great tit nestlings that were lighter than their average siblings, had shorter telomeres compared to the heavier ones. In pied flycatchers neither pollution nor growth stress were associated with telomere length, but adult females had significantly shorter telomeres compared to the nestlings. All the results related to redox status varied remarkably among the species and the age groups. In both species antioxidants were related to pollution. There were no significant associations between redox status and telomere length. Our results suggest that wild birds at a young age are vulnerable to pollution and growth stress induced telomere damage. Redox status seems to interact with pollution and growth, but more studies are needed to clarify the underlying physiological mechanisms of telomere attrition. Our study highlights that all the observed associations and differences between the sampling zones varied depending on the species, age, and degree of exposure to pollution.