Amyloid tracers binding sites in autosomal dominant and sporadic Alzheimer's disease

Amyloid imaging has been integrated into diagnostic criteria for Alzheimer's disease (AD). How amyloid tracers binding differ for different tracer structures and amyloid-β aggregates in autosomal dominant AD (ADAD) and sporadic AD is unclear.

Binding properties of different amyloid tracers were examined in brain homogenates from six ADAD with APPswe, PS1 M146V, and PS1 EΔ9 mutations, 13 sporadic AD, and 14 control cases.

³H-PIB, ³H-florbetaben, ³H-AZD2184, and BTA-1 shared a high- and a varying low-affinity binding site in the frontal cortex of sporadic AD. AZD2184 detected another binding site (affinity 33 nM) in the frontal cortex of ADAD. The ³H-AZD2184 and ³H-PIB binding were significantly higher in the striatum of ADAD compared to sporadic AD and control. Polyphenol resveratrol showed strongest inhibition on ³H-AZD84 binding followed by ³H-florbetaben and minimal on ³H-PIB.

This study implies amyloid tracers of different structures detect different sites on amyloid-β fibrils or conformations.