Dissolution Dynamic Nuclear Polarization capability study with fluid path.

Area of Science:

• Magnetic Resonance Imaging
• Hyperpolarization Techniques
• Medical Imaging Agents

Background:

• Low sensitivity in magnetic resonance imaging (MRI) necessitates signal enhancement methods.
• Dissolution Dynamic Nuclear Polarization (d-DNP) is a key hyperpolarization technique used clinically, particularly in cancer imaging.
• Ensuring a low bioburden in hyperpolarized agents is critical for patient safety, often achieved via closed fluid paths.

Purpose of the Study:

• To present a novel method for filling a closed fluid path for hyperpolarized agents, enabling its reuse.
• To evaluate the reproducibility of this filling method across different dosing scales (clinical and rodent studies).
• To assess the quality control parameters of the hyperpolarized agent produced using the new method.

Main Methods:

• Development of a reusable closed fluid path filling method.
• Application of the method using [1-13C]pyruvate as an example hyperpolarized agent.
• Investigation of reproducibility for high-dose (patient) and low-dose (rodent) applications.
• Analysis of six key quality control parameters.

Main Results:

• The developed filling method demonstrated high reproducibility.
• Standard deviations for quality control parameters were 3-10 times smaller than clinical acceptance criteria intervals.
• The method proved effective for both high-dose and low-dose applications.

Conclusions:

• The presented fluid path filling method allows for the reuse of components, potentially reducing costs and waste.
• High reproducibility of quality control parameters supports the clinical and preclinical utility of the method.
• This technique contributes to the reliable and safe application of hyperpolarized MRI agents.