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Published on: July 2, 2013
Anti-proteolytic activity of Ganoderma lucidum methanol extract against Pseudomonas aeruginosa
Nessma Ahmed El Zawawy1, Sameh Samir Ali
1Faculty of Science, Tanta University, Tanta, Egypt. dr.nessmaelzawawy@yahoo.com.
Introduction:
Protease enzyme is considered one of the most serious virulence factors produced by extended-spectrum β-lactamase-producing and multidrug-resistant Pseudomonas aeruginosa (ESβLMDRPA) clinical isolates.
Methodology:
The antibacterial activity of methanol extract of Ganoderma lucidum fruiting bodies was tested against a protease-producing ESβLMDRPA clinical isolate, showing its mode of action.
Results:
The extract showed high antibacterial activity. Its effect on purified protease indicated a reversible non-competitive protease inhibition (kis= 0.45 mg/mL).
Conclusions:
The G. lucidum extract could be a promising anti-proteolytic active against ESβLMDRPA. It may form a primary platform for further phytochemical studies and development of new drugs for therapy of skin burn infections.
Insights
Ganoderma lucidum extract shows potent antibacterial activity against multidrug-resistant Pseudomonas aeruginosa. This natural compound acts as a promising anti-proteolytic agent, potentially aiding in the development of new therapies for infections.
Area of Science:
- Microbiology
- Pharmacology
- Natural Product Chemistry
Background:
- Protease enzymes are critical virulence factors in extended-spectrum β-lactamase-producing and multidrug-resistant Pseudomonas aeruginosa (ESβLMDRPA) clinical isolates.
- ESβLMDRPA poses a significant threat due to its resistance to multiple antibiotics.
- Targeting virulence factors like proteases is a key strategy to combat resistant bacterial infections.
Purpose of the Study:
- To evaluate the antibacterial efficacy of Ganoderma lucidum methanol extract against a protease-producing ESβLMDRPA clinical isolate.
- To investigate the mode of action of the extract, specifically its effect on protease activity.
- To explore the potential of G. lucidum as a source for novel anti-infective agents.
Main Methods:
- Preparation of methanol extract from Ganoderma lucidum fruiting bodies.
- Assessment of antibacterial activity against ESβLMDRPA using standard microbiological assays.
- Enzyme inhibition assays to determine the effect of the extract on purified protease activity.
- Kinetic analysis to characterize the type and strength of protease inhibition.
Main Results:
- The Ganoderma lucidum methanol extract exhibited significant antibacterial activity against the tested ESβLMDRPA isolate.
- The extract demonstrated a reversible, non-competitive inhibition of the bacterial protease.
- The inhibition constant (kis) for protease inhibition was determined to be 0.45 mg/mL.
Conclusions:
- Ganoderma lucidum extract possesses promising anti-proteolytic properties effective against ESβLMDRPA.
- The findings suggest G. lucidum as a potential platform for developing new therapeutic agents.
- Further phytochemical investigation and drug development are warranted for treating infections, such as skin burns, caused by ESβLMDRPA.
