Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hemoglobin01:24

Hemoglobin

9.6K
Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well.
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
9.6K
Redox Reactions01:27

Redox Reactions

1.3K
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
1.3K
Redox Reactions01:24

Redox Reactions

59.2K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
59.2K
Oxygen Transport in the Blood01:27

Oxygen Transport in the Blood

7.9K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
7.9K
Redox Equilibria: Overview01:23

Redox Equilibria: Overview

1.7K
A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...
1.7K
Gene Families01:57

Gene Families

10.2K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
10.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metformin modulates the unfolded protein responses, altering lifespan and health-promoting effects in UPR-activated worms.

PloS one·2025
Same author

Large extension of Caenorhabditis elegans lifespan in diluted axenic medium: a balancing act between different survival responses.

The journals of gerontology. Series A, Biological sciences and medical sciences·2025
Same author

The Proprotein Convertase BLI-4 Is Required for Axenic Dietary Restriction Mediated Longevity in Caenorhabditis elegans.

Aging cell·2025
Same author

The Effect of Axenic Dietary Restriction on the Age-Related Changes in Caenorhabditis elegans.

The journals of gerontology. Series A, Biological sciences and medical sciences·2024
Same author

Mitochondrial H<sub>2</sub>O<sub>2</sub> release does not directly cause damage to chromosomal DNA.

Nature communications·2024
Same author

Reverse genetic screening during L1 arrest reveals a role of the diacylglycerol kinase 1 gene dgk-1 and sphingolipid metabolism genes in sleep regulation.

Genetics·2023
Same journal

Non-neuronal cell outgrowth in <i>C</i><i>. elegans</i>.

Worm·2017
Same journal

Chaperone-directed ubiquitylation maintains proteostasis at the expense of longevity.

Worm·2017
Same journal

Molting in <i>C. elegans</i>.

Worm·2017
Same journal

<i>sta-1</i> is repressed by <i>mir-58</i> family in <i>Caenorhabditis elegans</i>.

Worm·2017
Same journal

GExplore 1.4: An expanded web interface for queries on <i>Caenorhabditis elegans</i> protein and gene function.

Worm·2017
Same journal

SKN-1-independent transcriptional activation of glutathione S-transferase 4 (GST-4) by EGF signaling.

Worm·2017
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
06:10

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Published on: June 18, 2020

7.9K

Globin-based redox signaling.

Sasha De Henau1, Bart P Braeckman2

  • 1Biomedical Genetics, University Medical Center Utrecht , Utrecht, The Netherlands.

Worm
|October 4, 2016
PubMed
Summary
This summary is machine-generated.

Reactive oxygen species (ROS) act as cellular signals. A globin protein, GLB-12, in *C. elegans* generates ROS, contributing to redox signaling pathways and highlighting globins

Keywords:
globinreactive oxygen speciesredox signalingreproductionsuperoxide dismutase

More Related Videos

Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

12.6K
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
07:47

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator

Published on: October 20, 2021

3.4K

Related Experiment Videos

Last Updated: Mar 14, 2026

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
06:10

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Published on: June 18, 2020

7.9K
Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

12.6K
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
07:47

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator

Published on: October 20, 2021

3.4K

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Genetics

Background:

  • Reactive oxygen species (ROS) are increasingly recognized as crucial signaling molecules in cellular processes.
  • Globins, characterized by their redox-active heme iron, are well-positioned to engage in ROS metabolism.
  • Understanding the role of specific proteins in ROS signaling is vital for cellular communication research.

Purpose of the Study:

  • To investigate the involvement of the globin GLB-12 in a redox signaling pathway in *Caenorhabditis elegans*.
  • To elucidate the mechanisms by which GLB-12 produces and modulates ROS signals.
  • To explore the structural and biochemical properties of globins that facilitate their role in redox signaling.

Main Methods:

  • Observation of GLB-12's production of superoxide, a type of ROS.
  • Analysis of superoxide conversion to hydrogen peroxide gradients via superoxide dismutases.
  • Examination of regulatory mechanisms enhancing redox signal effectiveness.
  • Characterization of structural and biochemical properties of GLB-12 and other *C. elegans* globins.

Main Results:

  • GLB-12 was found to produce superoxide, initiating a ROS-mediated signaling cascade.
  • Superoxide is converted to hydrogen peroxide, forming a plasma membrane gradient.
  • Specific structural and biochemical attributes enable GLB-12 to perform redox reactions.
  • Similar properties were identified in two other *C. elegans* globins involved in redox biology.

Conclusions:

  • GLB-12 actively participates in a redox signaling pathway in *C. elegans*.
  • Globins involved in redox signaling share common structural and biochemical characteristics.
  • A subgroup of globins should be recognized for their vital role in redox signaling pathways.