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Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis
Liron Bar-El1, Yael Kalma1, Mira Malcov1
1IVF Lab and Wolfe PGD-Stem Cell Lab, Racine IVF Unit, Lis Maternity Hospital, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel Aviv, Israel.
Journal of Assisted Reproduction and Genetics
|October 4, 2016
Summary
Blastomere biopsy for preimplantation genetic diagnosis (PGD) significantly delays embryo development, impacting implantation rates. This affects key events like compaction and blastulation in cleavage-stage embryos.
Area of Science:
- Reproductive biology
- Embryology
- Genetics
Background:
- Preimplantation genetic diagnosis (PGD) involves embryo biopsy.
- The impact of biopsy on early embryonic development is not fully understood.
Purpose of the Study:
- To investigate the effects of blastomere biopsy for PGD on embryo dynamics, cleavage, development, and implantation.
- To analyze morphokinetic parameters of biopsied versus non-biopsied embryos.
Main Methods:
- Utilized the EmbryoScope™ time-lapse monitoring system for embryo culture.
- Analyzed 366 biopsied embryos and 385 control intracytoplasmic sperm injection (ICSI) embryos.
- EmbryoViewer™ was used to analyze time points of key embryonic events.
Main Results:
- Blastomere biopsy at ≥8 cells significantly delayed embryo compaction and blastulation compared to controls.
- Delays in preimplantation development were associated with affected postimplantation development.
- Comparison of implanted (KID positive) and non-implanted (KID negative) embryos revealed developmental differences.
Conclusions:
- Blastomere biopsy interferes with the dynamic sequence of embryonic developmental events.
- Biopsy leads to delayed compaction and blastulation, consequently decreasing implantation rates.
