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Related Concept Videos

Principles Of Column Chromatography01:13

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Chromatographic Methods: Terminology01:18

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Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
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High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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High-Performance Liquid Chromatography: Instrumentation00:57

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Elution Process01:05

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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High-Performance Liquid Chromatography: Types of Detectors01:15

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Hazards in chromatographic bioanalysis method development and applications.

Shirin Hooshfar1, Michael G Bartlett1

  • 1Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens, GA, USA.

Biomedical Chromatography : BMC
|October 4, 2016
PubMed
Summary
This summary is machine-generated.

This review addresses common errors in bioanalytical methods used for drug development. It offers practical strategies to avoid and manage hazards in quantitative determination of drugs and metabolites in biological samples.

Keywords:
application hazards in chromatographic bioanalysisbioanalysischromatographydevelopmenthazard

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Area of Science:

  • Pharmacology and Toxicology
  • Analytical Chemistry
  • Drug Development

Background:

  • Bioanalytical methods are crucial for quantifying drugs and metabolites in biological matrices throughout drug development.
  • Complex biological matrices present significant biological, chemical, and physical hazards that can compromise data quality.
  • Chromatographic techniques are commonly used in bioanalysis, but are susceptible to various errors.

Purpose of the Study:

  • To review common and significant errors encountered during bioanalytical method development, validation, and sample analysis.
  • To present practical approaches for mitigating and managing hazards in routine bioanalysis.
  • To enhance the reliability and accuracy of quantitative drug and metabolite determination.

Main Methods:

  • Literature review focusing on common errors in bioanalytical method development and validation.
  • Analysis of hazards encountered during the quantitative determination of drugs and metabolites in biological matrices.
  • Evaluation of strategies for error avoidance and hazard management in chromatographic bioanalysis.

Main Results:

  • Identification of frequent biological, chemical, and physical hazards impacting bioanalytical data quality.
  • Detailed discussion of errors during method development, validation, and sample analysis stages.
  • Presentation of practical solutions for managing and avoiding identified hazards in routine bioanalysis.

Conclusions:

  • Effective management of hazards during bioanalytical method development and validation is essential for reliable drug quantification.
  • Implementing practical strategies can significantly improve data quality and reduce errors in routine bioanalysis.
  • This review provides valuable insights for scientists involved in drug development and bioanalysis.