Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

17.6K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
17.6K
Mitochondrial Membranes01:45

Mitochondrial Membranes

2.2K
2.2K
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

4.9K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
4.9K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

13.6K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
13.6K
Membrane Lipids01:32

Membrane Lipids

35.2K
Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
35.2K
Membrane Lipids01:32

Membrane Lipids

15.9K
15.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sphingolipid regulation by yeast Mdm1 supports adaptive remodeling of the methionine transporter Mup1.

Molecular biology of the cell·2026
Same author

Sphingolipid regulation by yeast Mdm1 supports adaptive remodeling of the methionine transporter Mup1.

bioRxiv : the preprint server for biology·2026
Same author

Diet-induced phospholipid remodeling dictates ferroptosis sensitivity and tumorigenesis in the pancreas.

Cancer discovery·2026
Same author

SPNS2 exports sphingosine-1-phosphate and imports glucose.

Nature communications·2026
Same author

Metabolic plasticity of sphingolipids governs cancer cell fitness in acidic tumor ecosystems.

bioRxiv : the preprint server for biology·2026
Same author

An integrative approach to studying sphingolipid metabolism reveals p53 as a master regulator of the pathway.

Journal of lipid research·2026
Same journal

Moderate-altitude hypoxia is associated with attenuated diet-induced liver injury and coordinated carbon-metabolic and lipid remodeling.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same journal

The gut microbial lauroyl tryptamine antagonizes oxysterol-GPR183 signaling to modulate lipid-sensitive metabolic pathways in a sex-dependent manner.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same journal

HSF4 promotes lipid peroxidation by transcriptionally regulating ALOX15 during lens terminal differentiation.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same journal

Corrigendum to "Inducible beige adipocytes improve impaired glucose metabolism in interscapular BAT-removal mice" [Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1866 (2021) 158871].

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same journal

Caveolin-2 knockout suppresses the formation and progression of oral leukoplakia via lipid metabolism disruption.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same journal

Lipid droplet isolation as a novel platform for spectroscopic investigation of cargo modifications.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

Mitochondria-associated ER Membranes MAMs and Glycosphingolipid Enriched Microdomains GEMs: Isolation from Mouse Brain
10:32

Mitochondria-associated ER Membranes MAMs and Glycosphingolipid Enriched Microdomains GEMs: Isolation from Mouse Brain

Published on: March 4, 2013

25.2K

Sphingolipids in mitochondria.

María José Hernández-Corbacho1, Mohamed F Salama2, Daniel Canals1

  • 1Stony Brook Cancer Center and the Department of Medicine, Stony Brook University, Health Sciences Center, Stony Brook, NY 11794, USA.

Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids
|October 5, 2016
PubMed
Summary
This summary is machine-generated.

Sphingolipids, central to cell signaling, are key in cell growth and death. This review details ceramide metabolism, focusing on its mitochondrial roles in apoptosis and disease.

Keywords:
CancerCeramideMitochondrial apoptosisMitophagySphingolipid metabolism

More Related Videos

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays
08:27

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Published on: October 20, 2023

2.5K
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

4.7K

Related Experiment Videos

Last Updated: Mar 14, 2026

Mitochondria-associated ER Membranes MAMs and Glycosphingolipid Enriched Microdomains GEMs: Isolation from Mouse Brain
10:32

Mitochondria-associated ER Membranes MAMs and Glycosphingolipid Enriched Microdomains GEMs: Isolation from Mouse Brain

Published on: March 4, 2013

25.2K
Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays
08:27

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Published on: October 20, 2023

2.5K
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

4.7K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Medicine

Background:

  • Sphingolipids are bioactive lipids crucial for cell membrane signaling.
  • They regulate vital cellular processes including growth, senescence, differentiation, and apoptosis.
  • Dysregulated sphingolipid metabolism is implicated in various human diseases.

Purpose of the Study:

  • To provide a comprehensive overview of sphingolipid metabolism.
  • To highlight ceramide synthesis pathways, particularly mitochondrial generation and transport.
  • To elucidate the role of ceramide in mitochondrial function, apoptosis, mitophagy, and disease.

Main Methods:

  • Review of existing literature on sphingolipid metabolism.
  • Focus on biochemical pathways of ceramide synthesis.
  • Analysis of ceramide's role in mitochondrial-mediated apoptosis and mitophagy.

Main Results:

  • Sphingolipid metabolism is a complex, interconnected network with ceramide as a central hub.
  • Mitochondrial ceramide generation and transport are critical regulatory points.
  • Ceramide influences mitochondrial-mediated apoptosis and mitophagy.

Conclusions:

  • Understanding sphingolipid metabolism, especially ceramide's role in mitochondria, is vital for cellular function and disease insight.
  • Further research into ceramide pathways offers potential therapeutic strategies.
  • This review addresses current knowledge gaps and future research avenues.