Related Experiment Video
Updated: Mar 14, 2026
07:02
An Assay for Quantifying Protein-RNA Binding in Bacteria
Published on: June 12, 2019
7.1K
Characterization of RNA-Binding Proteins Using Protein Microarrays.
Guang Song1, Johnathan Neiswinger1, Heng Zhu2
1Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Cold Spring Harbor Protocols
|October 5, 2016
Summary
We developed a high-throughput method to quickly identify RNA-binding proteins (RBPs) that interact with specific RNA molecules. This protein microarray technique enables rapid RBP discovery for gene regulation studies.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- RNA-binding proteins (RBPs) are crucial regulators of gene expression.
- RBPs control cellular processes through interactions with RNA.
- Understanding these interactions is key for gene transcription and posttranscriptional regulation.
Purpose of the Study:
- To present a novel high-throughput method for identifying RNA-binding proteins (RBPs).
- To enable rapid discovery of specific RBPs that bind to a given RNA molecule.
- To utilize protein microarray technology for efficient RBP screening.
Main Methods:
- Development of a high-throughput protein microarray assay.
- Preparation of Cy5-labeled RNA probes.
- RNA probe denaturation and refolding.
- Performing RNA-binding assays on a yeast protein microarray.
Main Results:
- Successful implementation of a high-throughput method for RBP identification.
- Demonstration of rapid screening capabilities using protein microarray technology.
- Identification of specific RNA-protein interactions.
Conclusions:
- The presented method offers a rapid and efficient approach for identifying RNA-binding proteins.
- Protein microarray technology is effective for high-throughput screening of RNA-RBP interactions.
- This technique facilitates the study of gene regulation by RNA-binding proteins.
Related Concept Videos
02:24Ribosome Profiling
4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
02:34DNA Microarrays
21.8K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.8K
01:33Proteomics
10.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.1K