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Preparation of Yeast DNA Sequencing Libraries
Katja Schwartz1, Gavin Sherlock1
1Department of Genetics, Stanford University Medical School, Stanford, California 94305-5120.
Cold Spring Harbor Protocols
|October 5, 2016
Summary
This protocol details cost-effective DNA sequencing library preparation from yeast genomic DNA for Illumina sequencing. The method uses readily available reagents, reducing costs without compromising insert size flexibility for paired-end sequencing.
Area of Science:
- Molecular Biology
- Genomics
- Biotechnology
Background:
- DNA sequencing library preparation is crucial for genomic analysis.
- Commercial kits for library preparation can be expensive.
- Alternative, cost-effective methods are needed for wider accessibility.
Purpose of the Study:
- To describe a protocol for preparing yeast genomic DNA sequencing libraries.
- To provide a cost-effective alternative to commercial Illumina library preparation kits.
- To enable flexible insert size generation for paired-end sequencing.
Main Methods:
- Utilizes specific reagents primarily from New England BioLabs.
- Adapts standard molecular biology techniques for library construction.
- Includes steps for DNA shearing and size selection.
Main Results:
- Successfully generates DNA sequencing libraries from yeast genomic DNA.
- Significantly reduces the cost of library preparation compared to commercial kits.
- Allows for straightforward modification of insert sizes.
Conclusions:
- This protocol offers a budget-friendly approach to yeast DNA library preparation for Illumina sequencing.
- The method is adaptable for various insert sizes, enhancing its utility.
- Provides a valuable resource for researchers seeking to lower sequencing costs.
