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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Mar 14, 2026

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

High-Throughput Yeast Strain Sequencing.

Katja Schwartz¹, Gavin Sherlock¹

¹Department of Genetics, Stanford University Medical School, Stanford, California 94305-5120.

Cold Spring Harbor Protocols

|October 5, 2016

Summary

This summary is machine-generated.

High-throughput sequencing now makes yeast genome analysis accessible to most labs. This enables straightforward identification of genetic differences compared to the established reference genome.

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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Published on: March 22, 2018

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

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Last Updated: Mar 14, 2026

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

The first eukaryotic genome sequenced was yeast, with a high-quality 12 Mbp reference strain (S288c).
Advances in sequencing technology have made yeast strain sequencing a standard laboratory practice.
A high-quality reference genome with numerous updates facilitates strain comparison.

Purpose of the Study:

To introduce current high-throughput sequencing technologies.
To describe methods for analyzing sequencing data.
To facilitate the identification of differences in yeast strains.

Main Methods:

Utilizing high-throughput sequencing platforms.
Applying bioinformatic tools for data analysis.
Comparing sequencing data against the established yeast reference genome.

Main Results:

Yeast genome sequencing is now widely accessible.
Identification of genetic variations is feasible for non-repetitive regions.
Established reference genome aids in strain characterization.

Conclusions:

Modern sequencing technologies empower researchers to analyze yeast genomes routinely.
Comparative genomics of yeast strains is now more straightforward.
This accessibility accelerates research in yeast biology.