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MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
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Microarray analysis reveals altered circulating microRNA expression in mice infected with Coxsackievirus B3
Chaoyu Sun1, Lei Tong2, Wenran Zhao3
1Department of Cardiology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Experimental and Therapeutic Medicine
|October 5, 2016
Summary
This study reveals that peripheral blood microRNAs (miRNAs) change in mice infected with Coxsackievirus B3 (CVB3). These altered miRNAs may play a role in the development of inflammatory cardiomyopathy.
Area of Science:
- Virology
- Molecular Biology
- Cardiology
Background:
- Coxsackievirus B3 (CVB3) commonly causes inflammatory cardiomyopathy.
- The role of peripheral blood microRNAs (miRNAs) in CVB3-induced cardiomyopathy is not well understood.
Purpose of the Study:
- To investigate alterations in peripheral blood miRNA expression during CVB3 infection.
- To identify specific miRNAs differentially expressed in response to CVB3 infection over time.
Main Methods:
- Utilized miRNA microarray technology to compare miRNA expression profiles.
- Analyzed peripheral blood from CVB3-infected and normal mice at different time points (3 and 6 days).
- Validated differentially expressed miRNAs using quantitative polymerase chain reaction (qPCR) and predicted biological functions via bioinformatics analysis.
Main Results:
- Identified 96 differentially expressed miRNAs at 3 days post-infection and 89 at 6 days post-infection.
- qPCR validation confirmed the microarray findings, showing consistency in differential miRNA expression.
- Bioinformatics analysis predicted potential roles for these miRNAs in hypertrophic, dilated, and arrhythmogenic right ventricular cardiomyopathies.
Conclusions:
- Peripheral blood miRNA expression is significantly altered during CVB3 infection.
- These changes are dynamic and vary with infection duration.
- The identified miRNAs may offer insights into the pathogenesis of CVB3-related cardiomyopathies.
