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Low Proteolytic Clipping of Histone H3 in Cervical Cancer

Jorge Sandoval-Basilio1, Nicolás Serafín-Higuera2, Octavio D Reyes-Hernandez3

  • 1Área de investigación clínica, Unidad de Innovación Clínica y Epidemiológica de la Secretaría de Salud del estado de Guerrero, Acapulco, Gro., México.; Laboratorio de Biología Molecular, Universidad Hipócrates, Acapulco, Gro., México.

Journal of Cancer
|October 5, 2016
PubMed

Insights

Histone H3 clipping, a gene expression regulator, is reduced in cervical cancer (CC). This process, involving specific proteases, suggests a role in CC

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Gene expression in cervical cancer (CC) is influenced by chromatin modifications.
  • Proteolytic clipping of histone H3 is a recently identified gene expression regulator.
  • The role of histone H3 clipping in CC has not been previously investigated.

Purpose of the Study:

  • To investigate the mechanism of histone H3 clipping in cervical cancer.
  • To determine if histone H3 clipping occurs in CC and how it differs from normal tissue.

Main Methods:

  • Utilized HeLa cells as a model for CC.
  • Analyzed human CC tissue samples and compared them with control tissues.
  • Identified proteases responsible for histone H3 clipping in HeLa cells.

Main Results:

  • Histone H3 cleavage was found to be significantly lower in CC tissues compared to control tissues.
  • Serine and aspartyl proteases were identified as the enzymes catalyzing histone H3 clipping in HeLa cells.

Conclusions:

  • Histone H3 clipping is a process that occurs in CC.
  • The reduced histone H3 clipping in CC suggests its involvement in the disease's post-translational modification system.
Keywords:
Cervical cancerHeLa.Histone 3Histone H3 clippingHistone modificationsHistone proteolysis

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