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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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Microdose fluorescence imaging of ABY-029 on an operating microscope adapted by custom illumination and imaging

Jonathan T Elliott1, Alisha V Dsouza1, Kayla Marra1

  • 1Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA.

Biomedical Optics Express
|October 5, 2016
PubMed

Insights

A new near-infrared fluorescence imaging system enhances surgical oncology by enabling picomolar sensitivity for tumor-specific agents. This custom device is suitable for microdose phase 0 clinical trials, improving tumor detection during surgery.

Area of Science:

  • Surgical Oncology
  • Medical Imaging
  • Biotechnology

Background:

  • Fluorescence-guided surgery shows promise in oncology but is limited by current imaging technology.
  • Existing systems lack the sensitivity or portability needed for novel tumor-specific agents.
  • The microdose pathway offers new agents but requires advanced detection methods.

Purpose of the Study:

  • To develop a highly sensitive near-infrared fluorescence imaging system for surgical oncology.
  • To evaluate the system's performance with tumor-specific agents in preclinical models.
  • To assess the device's suitability for microdose (Phase 0) clinical trials.

Main Methods:

  • A custom illumination and imaging module was integrated with a commercial operating microscope.
  • The system's limits of detection and specifications were rigorously characterized.
  • In vivo efficacy was tested in a rat orthotopic glioma model using ABY-029 after microdose administration.

Main Results:

  • The developed system achieves picomolar sensitivity for near-infrared fluorescence imaging.
  • System specifications were defined, demonstrating its advanced capabilities.
  • The device successfully detected ABY-029 in a rat glioma model, validating its in vivo performance.

Conclusions:

  • The custom-built fluorescence imaging module significantly enhances surgical oncology capabilities.
  • The system's high sensitivity and portability make it ideal for utilizing microdose agents.
  • This technology is well-suited for microdose Phase 0 clinical trials, advancing precision surgery.
Keywords:
(170.1610) Clinical applications(170.3880) Medical and biological imaging(170.4730) Optical pathology(260.2510) Fluorescence

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