[Multilocus Sequence Typing analysis of human Campylobacter coli in Granada (Spain)]
J A Carrillo-Ávila, A Sorlózano-Puerto, M Pérez-Ruiz
1José Gutiérrez-Fernández, Departamento de Microbiología. Facultad de Medicina. Avenida de la Investigación 11. E-18016 Granada, Spain. josegf@go.ugr.es.
Objective:
Different subtypes of Campylobacter spp. have been associated with diarrhoea and a Multilocus Sequence Typing (MLST) method has been performed for subtyping. In the present work, MLST was used to analyse the genetic diversity of eight strains of Campylobacter coli.
Methods:
Nineteen genetic markers were amplified for MLST analysis: AnsB, DmsA, ggt, Cj1585c, CJJ81176-1367/1371, Tlp7, cj1321-cj1326, fucP, cj0178, cj0755/cfrA, ceuE, pldA, cstII, cstIII. After comparing the obtained sequences with the Campylobacter MLST database, the allele numbers, sequence types (STs) and clonal complexes (CCs) were assigned.
Results:
The 8 C. coli isolates yielded 4 different STs belonging to 2 CCs. Seven isolates belong to ST-828 clonal complex and only one isolate belong to ST-21. Two samples came from the same patient, but were isolated in two different periods of time.
Conclusions:
MLST can be useful for taxonomic characterization of C. coli isolates.
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